1887

oa South African Journal of Clinical Nutrition - Assessment of body composition in lactating mothers in a rural African community using deuterium oxide

Volume 17, Issue 1
  • ISSN : 1607-0658

 

Abstract

The deuterium oxide (DO) dilution method for measuring body composition was validated against the widely used skinfold (SF) measurement-based equation of Durnin and Womersley. The study involved 10 lactating women living in a rural community in Nandi, Kenya and participating in a cross-sectional study aimed at determining their iron and vitamin A status.


The selection criteria were exclusive breast-feeding, infants between 2 and 4 months of age,maternal parity <4, birth weight more than 2 500 g and no congenital abnormalities. Maternal and infant-anthropometric measurements were taken.DO (approximately 0.1 g 2HO/kg body water) was given orally to each mother accordingly to the Maastricht protocol (DO) for total body water (TBW) determination. The 2H enrichment of the urine was measured using gas-isotope-ratio mass spectrometry. Blood samples were collected. Serum retinol and ferritin were determined using high- performance liquid chromatography (HPLC) and enzyme-linked immunosorbent assay (ELISA) respectively. Body mass index (BMI) was determined as weight/height (m2).The Bland-Altman pair-wise comparison was used to compare maternal fat-free mass (FFM), body fat (BF)and percentage body fat (% BF) that were determined based on the DO and SF techniques.
Maternal mean (± standard deviation (SD)) for parity, age, BMI, haemoglobin (Hb), serum ferritin and serum retinol were 3 (2), 26 (4) years, 23.4 (4), 12.1 (1.8) g/dl, 10.3 (4.0) µg/l and 0.696 (0.300) µmol/l respectively. The FFM, BF and % BF accordingly to the DO and SF methods respectively were 44.0 (4.7)kg v. 42.9 (5.9) kg, 16.7 (8.8) kg v. 17.8 (7.5) kg and 26.2 (8.1)% v. 28.4 (6.4)%. Limits of agreement for underestimation of FFM and % BF were 4.4 kg (SEE 3.4) and 11.6% (SEE 5.8) respectively. Bias in the measurement of FFM and % BF was 1.1 kg (SEE 1.9), and -2.2% (SEE 3.3) respectively.
The variability (5.8 - 17.5%) observed in the SF technique may result in lower prediction of% BF. This may be an important factor for community-based nutritional interventions that aim at improving the body composition of vulnerable groups such as pregnant and lactating women or subjects with severe undernutrition.

Loading full text...

Full text loading...

Loading

Article metrics loading...

/content/m_sajcn/17/1/EJC64849
2004-02-01
2016-12-10

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error