oa Molecular Diagnosis and Vaccines - Detection of Chlamydia pneumoniae in muman atherosclerotic plaques by polymerase chain reaction
Chlamydia pneumoniae (C. pneumoniae) has recently been implicated in the development of atherosclerosis. The aim of work was to investigate the role of C. pneumoniae in the pathogenesis of atherosclerotic plague. Endarterectomy and vascular wall specimens from seventy-five patients (mean age, 59 10; 55 male, 20 female) were studied. They included 50 specimens from patients with ischemic vascular disease (20 patients with coronary artery bypass grafting, 18 patients with carotid endarterectomy, and 12 patients with surgery of the abdominal aorta for atherosclerotic obstructive lesions), and 25 control specimens from healthy regions of the ascending aorta from non ischemic patients. The presence of C. pneumoniae DNA in endarterectomy specimens was assessed by nested polymerase chain reaction (PCR). Positive PCR was found in 14 (28%) of the 50 atherosclerotic plaque specimens but in none of the healthy vascular-wall specimens (P < 0.001). These findings support the idea that C. pneumoniae might has a role in the development of atherosclerosis in Egypt, where infection is prevalent and where conventional risk factors fail to explain the high prevalence of atherosclerotic vascular disease.
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