oa Molecular Diagnosis and Vaccines - Characterization of Mycobacterium tuberculosis complex isolates from sputum of patients in Cairo, Egypt
We characterized 45 (11 multi-drug resistant MDR and 34 drug susceptible) Mycobacterium tuberculosis complex (MTC) isolates from sputum samples of Egyptian patients with pulmonary tuberculosis. One M. bovis and 44 M. tuberculosis (MTB) isolates were identified by allele-specific PCR analysis of the oxyR gene. Among the 44 M. tuberculosis isolates, 36 unique strain types (from 5-16 copies of IS6110) and 4 clusters (with 2 isolates each) were found by IS6110 restriction fragment length polymorphism (RFLP) analysis. None of the 11 MDR isolates were clustered. 22 (50 %) were in spoligotype clusters compared to the 8 out of 44 isolates (18.18%) in RFLP clusters. The spoligotypes included at least 5 that have been previously reported from other countries and from other studies in Egypt. We demonstrated evidence of diversity particularly -resistant strains consistent with low-incidence countries.
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