n Medical Technology SA - The growth response of 0111 subjected to sublethal high hydrostatic pressure : peer reviewed original article

Volume 23, Issue 2
  • ISSN : 1011-5528


O111, in peptone water, was subjected to high hydrostatic pressure (HHP) of 200 MPa for 15 minutes at 8 and 50 °C. Sub-culturing was performed and growth responses evaluated at 0, 6, 18, 24, 30, 42 and 48 hours. Percentage differences between test and control cells were calculated and results showed that O111 was only sublethally injured and able to repair itself after incubation in an enrichment broth. Sub lethally injured microorganisms could still present a potential threat given that they are able to repair themselves under suitable conditions. It is advisable to consider enrichment for at least 18 hours prior to selective cultivation. The concentration of bacteria would also dictate the magnitude of the HHP being applied. Further studies should be conducted to assess the virulence of post-HHP O111, as metabolic adaptation and cell repair might also have an impact on the lipopolysaccharide (LPS) toxin structure, the ability to form biofilm, as well as the accuracy of serological assessment.

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